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Given the importance of mixing and mass transfer in fermentation and cell culture processes and the potentially huge literature available on the subject, it is not possible to cover all aspects of this topic within the scope of this chapter. What are considered the most important subject matters are addressed here. In Section 18-2 we focus on the aspects of scaling up and scaling down fermentation processes. Although only microbial and fungal systems are considered, similar principles can be applied for cell culture processes. Scale-up of industrial fermentation processes occurs either when a new process is scaled up or when an existing process is subjected to modi cations (e.g., media or strain improvements). Since scale-up is still largely performed using empirical knowledge and although scale-up can sometimes be successful, it is dif cult to use it for optimization purposes. A more rapid, process-speci c approach that is capable of predicting the performance with greater con dence on scale-up is desired. The limitations of traditional scale-up methods have been highlighted, and alternative methods using a scaledown approach are described in detail. Only stirred tank bioreactors have been considered in this discussion since the use of such systems is overwhelmingly dominant in the fermentation and cell culture industries. A signi cant part of this chapter is devoted to a description of studies that have measured spatial variations in dissolved oxygen, substrate, and pH at large scales of operation and

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Index fields, also called key fields, become an absolute necessity when two or more tables or flat-file databases are combined to create a relational database. As the name implies, a relational database presents related information. For example, suppose that you create another table called BookSales to accompany the previous book database example:

Horst Feistel (Courtesy of IBM).

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(5.24)

The stereoregularity of propylene polymerization is strongly related to the geometry of the catalytic centers and the orientation of complexation 05,306

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Figure 7.30 Illustration of Step 1 and Step 2 of enzymic surface mechanism of LysU catalyzed diadenosine-5 , 5 -P 1 ,P 4 -tetraphosphate (Ap4 A) 4 formation (see s 6 and 8). (a) Ribbon display structure illustration of LysU monomer with bound lysyladenylate intermediate 3 and pyrophosphate rendered in stick display mode (orange: AMP residue; green: lysyl residue; light blue: pyrophosphate). Mg+2 ions are rendered as Van der Waals spheres (purple). (b) Active site close-up of LysU monomer structure shown in (a) (pdb: 1e1t).

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industrial route, is now of limited use. Because of the high energy requirements of acetylene production, this process has been largely replaced by the ethylene chlorination-oxychlorination reaction (see Section 6.3.4). The catalytic hydrochlorination of acetylenel88-190 demands high-quality reactants. Acetylene must be free of catalyst poisons (S, P, As), and HCl must not contain chlorine. Since water reacts with the product vinyl chloride to give acetaldehyde and HCl, the reactants must be dry. In the more widely used vapor-phase synthesis, the premixed reactants with a slight excess of HCl are fed into a multitube fixed-bed reactor kept at about 200 C. The catalyst is almost invariably the superior mercury (II) chloride supported on active carbon. Certain additives (Ce, Th, and Cu chlorides) are used to suppress the loss of the volatile HgCh. High conversion (near 100%) and high selectivity (98%) are achieved. The addition of a second molecule of HCl to vinyl chloride may produce 1,I-dichloroethane, the only significant byproduct.

Hyperlinks panel (choose Window Interactive Hyperlinks or Window Type & Tables Cross-References): The Hyperlinks panel lets you set up hyperlinks from and to document objects and pages, as well as to World Wide Web locations. It also lets you create cross-references for use in automatic text. Bookmarks panel (choose Window Interactive Bookmarks): The Bookmarks panel creates the bookmarks used in PDF files to create clickable indexes and tables of contents. Buttons panel (choose Window Interactive Buttons): The Buttons panel sets pushbutton actions for PDF and Flash files. Page Transitions panel (choose Window Interactive Page Transitions): The Page Transitions panel sets page-transition effects for PDF and Flash files. Animation panel (choose Window Interactive Animation): The new Animation panel lets you set up and manage animations for Flash files. Timing panel (choose Window Interactive Timing): The new Timing panel lets you assign the times for various animated objects display and actions. Object States panel (choose Window Interactive Object States): The new Object States panel lets you assign and manage states for objects, for use with interactive Flash documents. Media panel (choose Window Interactive Media): The new Media panel lets you access and manage video files for PDF and Flash files. Preview panel (choose Window Interactive Preview): The new Preview panel lets you preview how PDF files, Flash files, and Web pages work including their interactive objects and animations while you are creating them, before exporting them as final files.

lyotropic mesophases have been summarised (Table 1.4) using the Luzzati nomenclature. Structures and transitions between mesophases are very relevant to the biological behaviour of lipids and so these will be discussed. However, given the current state of knowledge on lipids and their macromolecular assemblies, there can be no absolute certainty concerning precisely which of the lyotropic mesophases are actually relevant in biology and which are not.

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